Flocculating Activity of a Bioflocculant from Bacillus megaterium BMBF in Treatment of Domestic and Coal Mine Wastewater

Attention has been paid to bioflocculants production because of their effectiveness, innocuousness and environmental friendliness. This study aimed characterise a bioflocculant from Bacillus megaterium BMBF apply it in wastewater treatment. The proteins, carbohydrates uronic acid were calculated using the Bradford, phenol–sulphuric carbazole assays, respectively. An energy-dispersive X-ray (EDX) infrared spectrometry employed for identification elemental composition effective units, Cytotoxicity was carried out against Vero (African green monkey kidney) bovine dermis cells colorimetric cytotoxicity assay. reduction chemical oxygen demand (COD) biological (BOD) domestic coal mine studied Jar test. flocculant composed 12% protein, 27% 61% acid. Infrared indicated hydroxyl, carboxyl amino groups. EDX C (61%) O (17.5%) as main elements. revealed mean inhibition concentration 59 ug/mL 240 µg/mL on cells. Maximum COD BOD removal percentages 97% 99.3% recorded treatment about 99.2% 93% wastewater. In conclusion, B. potential industrial utility.